Capture of enzyme aggregates by covalent immobilization on solid supports. Relevant stabilization of enzymes by aggregation

Abstract In this paper, a novel procedure for the immobilization and stabilization of enzymes is proposed: multipoint covalent attachment bi-molecular enzyme aggregates. This protocol allows “capture” fixation aggregate on support surface. addition to by attachment, aggregation promotes very interesting stabilizing effects. presence low concentrations polyethylene glycol (30 %) dimeric amine oxidase from Pisum sativum forms soluble Enzyme aggregates were analyzed Dynamic Light Scattering full chemical loading mesoporous (10 % agarose gels activated with glyoxyl groups). The was immobilized glyoxyl- at pH 8.5 though four amino termini two molecules (Lys residues are not reactive pH). aggregated structure cannot undergo any movement (translational or rotational) after “fixed” surface even removal PEG. further incubated 10 in order allow Lys react groups support. has an important effect stabilization: derivative 40 fold more stable than similar isolated 200 native experiments thermal inactivation.